Pricing Overview
We have experience with a wide range of techniques and the ability to accommodate a variety of projects. As a guide, we have listed pricing below for some of the more common types of sequencing runs. To obtain a quote or discuss a project type that is not listed here, please contact us.
Our bulk RNAseq and miRNAseq pricing includes library prep and sequencing as described. Our DNA library prep pricing only includes library prep, as the required sequencing depth will be project specific. Our nucleic acid extraction services are also listed further down the page.
| Bulk Sequencing Service | Sequencing Configuration | <32 Samples | 32+ Samples |
| mRNAseq (polyA selection) | 18-20 M reads/sample, 100PE | $370 | $330 |
| Total RNAseq | 25-30 M reads/sample, 100PE | $400 | $360 |
| Paxgene RNAseq (+globin depletion) | 25-30 M reads/sample, 100PE | $530 | $480 |
| miRNAseq | 10-15 M reads/sample, 100PE | $440 | $400 |
| DNA library prep | Library prep only | $90 | $80 |
Our single cell library prep and sequencing services are priced separately as the number of cells captured will affect the required amount of sequencing. Our standard gene expression assay is run using the 5' 10X Genomics method, and the VDJ and Feature Barcode library preps are available as add-on services to a gene expression capture.
| Single Cell/Nuclei Capture & Library Prep | Pricing Unit | Unit Cost |
| Gene Expression Capture & Library Prep (Next GEM) | Price/capture (up to 10,000 cells) | $1,900 |
| Gene Expression Capture & Library Prep (GEM-X) | Price/capture (up to 20,000 cell) | $1,700 |
| VDJ Library Prep (Next GEM or GEM-X) | Price/capture | $300 |
| Feature Barcode Library Prep (Next GEM or GEM-X) | Price/capture | $300 |
| ATAC Capture & Library Prep | Price/capture (up to 10,000 nuclei) | $2,000 |
| Multiome (ATAC + Gene Expression) Capture & Library Prep | Price/capture (up to 10,000 nuclei) | $3,600 |
| Flex Singleplex Capture & Library Prep | Price/capture (up to 10,000 cells) | $2,100 |
| Flex Multiplex (4BC) Capture & Library Prep | Price/capture (up to 40,000 cells) | $4,000 |
| Flex Multiplex (16BC) Capture & Library Prep | Price/capture (up to 128,000 cells) | $9,200 |
| Nuclei Isolation | Cost/Sample |
| Nuclei Isolation | $240 |
| Library Sequencing | Pricing Unit | Unit Cost |
| Sequencing of Gene Expression Libraries | Price/cell (50,000 reads/cell) | $0.24 |
| Sequencing of VDJ or Feature Barcode Libraries | Price/cell (5,000 reads/cell) | $0.03 |
| Sequencing of ATAC Libraries | Depends on project | |
| Sequencing of Flex Fixed RNA Profiling Libraries | Price/cell (25,000 reads/cell) | $0.12 |
We offer spatial transcriptomics using the 10x Genomics CytAssist Platform. RNA quality assessment of FFPE tissue blocks or archived tissue slides is required, and we will only accept samples with a DV200 score that is >30%. If starting from FFPE tissue blocks, you are required to work with a histology or imaging core for the upstream steps before transferring the tissue slides to us.
| Spatial Transcriptomics | Pricing Unit | Unit Cost |
| RNA extraction (Qiagen RNeasy FFPE) and DV200 determination | Price/Sample from tissue slide or FFPE tissue block | $32 |
| Visium HD CytAssist library prep | Price/Visium HD slide (with two 6.5mm x 6.5mm areas) | $6,800 |
| Sequencing of Visium HD libraries | Price/1% of capture area (275 M reads for 100% covered) | $14 |
If you would prefer to prepare your own libraries, we also offer a variety of sequencing options. User-prepared libraries must be run separately in their own lane or flowcell.
| Run Type | NovaSeq Flowcell | Expected Reads/Run | Cost/Run |
| 100 cycles (100SR, PE50) | SP | 0.65-0.8 billion reads | $3,500 |
| S1 | 1.3-1.6 billion reads | $5,500 | |
| S2 | 3.3-4.1 billion reads | $9,800 | |
| 200 cycles (PE100, PE75) | SP | 0.65-0.8 billion reads | $4,600 |
| S1 | 1.3-1.6 billion reads | $7,100 | |
| S2 | 3.3-4.1 billion reads | $12,500 | |
| S4 | 8-10 billion reads | $18,100 | |
| S4 (single lane) | 2-2.5 billion reads | $4,600 | |
| 300 cycles (PE150) | SP | 0.65-0.8 billion reads | $5,300 |
| S1 | 1.3-1.6 billion reads | $8,000 | |
| S2 | 3.3-4.1 billion reads | $14,100 | |
| S4 | 8-10 billion reads | $20,700 | |
| 500 cycles (PE250) | SP | 0.65-0.8 billion reads | $7,500 |
| MiSeq Run Type | Expected Reads/Run | Cost/Run |
| MiSeq v3, 600 cycle kit | Up to 25 million reads | $2,500 |
| MiSeq v3, 150 cycle kit | Up to 25 million reads | $1,400 |
| MiSeq v2, 500 cycle kit | Up to 15 million reads | $1,900 |
| MiSeq v2, 300 cycle kit | Up to 15 million reads | $1,600 |
| MiSeq v2, 50 cycle kit | Up to 15 million reads | $1,200 |
| MiSeq v2 Micro, 300 cycle kit | Up to 4 million reads | $850 |
| MiSeq v2 Nano, 500 cycle kit | Up to 1 million reads | $800 |
| MiSeq v2 Nano, 300 cycle kit | Up to 1 million reads | $600 |
These prices include QC via Qubit and TapeStation or Fragment Analyzer. *Please note that extraction projects have a six sample minimum.
| RNA Extraction Services | Starting Sample Type | Cost/Sample |
| Qiagen RNeasy Mini* | Cells/tissue snap frozen or in RLT | $24 |
| Qiagen RNeasy Micro* | Cells/tissue snap frozen or in RLT | $29 |
| Qiagen miRNeasy Mini/Micro* | Cells/tissue snap frozen or in QIAzol | $26 |
| MagMAX PAXgene Blood RNA* | Blood in PAXgene RNA tubes | $26 |
We are happy to provide quality control services for various samples that you have prepared yourself. **Please note that Bioanalyzer projects have a six sample minimum.
| QC Service Type | Cost/Sample |
| Bioanalyzer (RNA Nano or RNA Pico)** | $7 |
| Bioanalyzer (High Sensitivity DNA)** | $10 |
| TapeStation (RNA, RNA-HS, D5000-HS, D1000-HS, Genomic DNA) | $8 |
| Fragment Analyzer (RNA, RNA-HS, DNA-HS NGS Fragment (1-6000bp)) | $6 |
| Qubit (DNA or RNA) | $3 |
| Library QC, pooling and normalization | $14 |
We offer a variety of bioinformatics services to support data generated in our genomics core. This can include a basic alignment and annotation of your sequencing data to produce gene count tables and bam files, or further statistical analyses to identify differentially expressed genes and activated pathways. As the scope of each project can vary widely, we encourage you to contact us for a quote.
Last Updated: 2024-08-30