Next Generation Sequencing

We offer next generation sequencing services on our Illumina NovaSeq 6000 and Illumina MiSeq. The combination of these systems provides us with a wide degree of flexibility in terms of read length and depth, allowing us to quickly sequence small amplicon-based sequencing projects as well as larger whole-genome scale projects.

Overview of Illumina Sequencing by Synthesis Workflow

NovaSeq 6000

The NovaSeq combines throughput and flexibility for virtually any method, genome, and scale; supporting diverse applications such as large whole-genome sequencing, exome and large panel sequencing, single-cell profiling, transcriptome sequencing, and more. It utilizes a variety of flow cell types (SP, S1, S2, S4) to provide the broadest output range. The NovaSeq 6000 System generates from 80Gb/800M reads to 3Tb/10B reads of data in single flow cell mode. In dual flow cell mode, output can be up to 6Tb and 20B reads.

Flow Cell TypeSPS1S2S4
Lanes per Flow Cell2224
Clusters PF0.65 – 0.8 billion1.3 – 1.6 billion3.3 – 4.1 billion8 – 10 billion
Available Run Configurations
PE50, SR100 (100 cycle)YesYesYes
PE100 (200 cycle)YesYesYesYes
PE150 (300 cycle)YesYesYesYes
PE250 (500 cycle)Yes


The MiSeq is a smaller unit used primarily for small whole-genome sequencing, targeted gene (amplicon) sequencing, immune repertoire sequencing, and 16s metagenomics sequencing. We also offer a variety of flow cell types for the MiSeq to better serve a range of sequencing depths and project sizes.

Flow Cell Typev2 Nanov2 Microv2v3
Clusters PFUp to 1 millionUp to 4 millionUp to 15 millionUp to 25 million
Available Run Configurations
PE75, SR150 (150 cycle)Yes
PE150 (300 cycle)YesYesYes
PE250 (500 cycle)YesYes
PE300 (600 cycle)Yes